The research of the Bioanalysis group of the Department of Chemical Engineering and Analytical Chemistry of the Faculty of Chemistry of the University of Barcelona is mainly focused on the development and application of high resolution separation techniques based on mass spectrometry to solve complex analytical problems related to organic sciences (directed or undirected) applied to biomedicine, the pharmaceutical industry, and the agri-food industry. The group is particularly interested in the improvement of sample pretractament, separation, characterization, sensitivity and reproducibility in the analysis of peptides, proteins, glycoproteins, protein oligomers, metabolites, microRNAs and other microRNAs, microRNAs and other bioactive elements and compounds in biological samples, biopharmaceuticals, foods and their residues using LC-MS, CE-MS and related techniques, as well as in the interpretation of the data generated using chemometric multivariate data analysis tools. These analyses have a great applicability in the quality control of food and its contaminants, the authentication and prevention of fraud, the development of functional foods and nutraceuticals, the evaluation of the effects of food components on health, and the detailed characterization of waste from the agri-food industry for its valorization.

Fernando Benavente
Investigador principal
Fernando Benavente

Fernando Benavente graduated in Chemical Sciences in 1997, completed an experimental Master in Analytical Chemistry in 1998 and obtained his PhD degree in Chemical Sciences in 2003 at the University of Barcelona. Since 2021 he is Professor in the Department of Chemical Engineering and Analytical Chemistry of the UB. He has been a member of this group since 1997, but has also carried out doctoral and postdoctoral research stays at The RW Johnson Pharmaceutical Research Institute (Raritan, New Jersey, USA), the National University of Rosario (Rosario, Argentina), the University of Leiden (Leiden, The Netherlands), the National University of La Plata (La Plata, Argentina) and the University of Ottawa (Ottawa, Canada). She also belongs to the Institute of Nutrition and Food Safety of the UB (INSA-UB) and to the teaching innovation group recognized by the UB ‘Analytical Chemistry in Experimental Sciences and Health Sciences’. His research contributions include more than 120 publications, most of them in journals indexed in the first quartile of his areas of expertise, several book chapters and more than 200 presentations at national and international conferences. In 2014 The Analytical Scientist magazine included him in its ‘top 40 under 40’. He is currently a member of the board of the Spanish Society of Analytical Chemistry (SEQA) and editor of the journals Foods (MDPI, Q1) and Actualidad Analítica (SEQA).

https://orcid.org/0000-0002-1688-1477
Equip
Línies de recerca
  • Development of analytical methodology for protein, glycoproteomics, metabolomics and targeted and untargeted miRNA research.
  • Characterization of new protein sources, allergenic proteins and food intolerances.
  • Characterization of bioactive elements and compounds and their metabolites in food and their residues.
  • Biomonitoring the effects of food components and exposure to their contaminants on human health.

Galindo-Luján, R; Pont, L; Sanz-Nebot, V; Benavente, F

Protein profiling and classification of commercial quinoa grains by MALDI-TOF-MS and chemometrics

Food Chemistry, 398 (2023) 133895; DOI: https://doi.org/10.1016/j.foodchem.2022.133895

Vergara-Barberán, M; Simó-Alfonso, EF; Herrero-Martínez, JM; Benavente, F

Accurate determination of the milk protein allergen beta-lactoglobulin by on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry

Talanta 259 (2023) 124542; DOI: https://doi.org/10.1016/j.talanta.2023.124542

Ghafoori, Z; Tehrani, T.; Pont, L; Benavente, F

Separation and characterization of bovine milk proteins by capillary electrophoresis-mass spectrometry

Journal of Separation Science, 45 (2022) 3614-3623; DOI: https://doi.org/10.1002/jssc.202200423

Salim, H; Pero-Gascon, R; Giménez, E; Benavente, F

On-line coupling of aptamer affinity solid-phase extraction and immobilized enzyme microreactor capillary electrophoresis-mass spectrometry for the sensitive targeted bottom-Up analysis of protein biomarkers

Analytical Chemistry, 94 (2022) 6948-6956; DOI: https://doi.org/10.1021/acs.analchem.1c03800

Manz, C; Mancera-Arteu, M; Zappe, A; Hanozin, E; Polewski, L; Giménez, E; Sanz-Nebot, V; Pagel, K

Determination of Sialic Acid Isomers from Released N-Glycans Using Ion Mobility Spectrometry

Analytical Chemistry, 94 (2022) 13323-13331; DOI: https://doi.org/10.1021/acs.analchem.2c00783

Galindo-Luján, R; Pont, L; Minic, Z; Berezovski, MV; Sanz-Nebot, V; Benavente, F

Characterization and differentiation of quinoa seed proteomes by label-free mass spectrometry-based shotgun proteomics

Food Chemistry, 363 (2021) 130250; DOI: https://doi.org/10.1016/j.foodchem.2021.130250

Pont, L; Kuzyk, V; Benavente, F; Sanz-Nebot, V; Mayboroda, OA; Wuhrer, M; Lageveen-Kammeijer, GSM

Site-specific N‑linked glycosylation analysis of human carcinoembryonic antigen by sheathless capillary electrophoresis-tandem mass spectrometry, Journal of Proteome Research

Journal of Proteome Research, 20 (2021) 1666-1675; DOI: https://dx.doi.org/10.1021/acs.jproteome.0c00875

Pero-Gascon; R; Benavente; F; Minic; Z; Berezovski, MV; Sanz-Nebot, V

On-line aptamer affinity SPE-CE-MS for the analysis of blood alpha-synuclein

Analytical Chemistry, 92 (2020) 1525-1533; DOI: http://dx.doi.org/10.1021/acs.analchem.9b04802

Junza, A; Saurina, J; Minguillón, C; Barrón, D

Metabolites in milk after enrofloxacin treatment and their persistence to temperature

Journal of Agricultural and Food Chemistry, 70 (2022) 8441-8450; DOI: https://doi.org/10.1021/acs.jafc.2c02230

FRI-INSA-2023/1, Evaluación in vivo de la biodisponibilidad de los polifenoles contenidos en diferentes variedades de quinoa

L. Pont (UB)

12/2023-12/2024

Institut d’investigació en nutrició i recerca alimentària (INSA-UB)

CEX2021-001234-M, Institut d’investigació en nutrició i seguretat alimentària (INSA-UB)

RM Lamuela (UB)

01/2023-12/2024

Ministerio de Ciencia, Innovación y Universidades

BioA-UB/CIM-Cuba, Conveni de col·laboració per a la realització d'una recerca entorn a la NeuroEPO i la rhEPO

E. Giménez / V. Sanz (UB)

01/2023-01/2027

PID2021-127137OB-I00, Proteómica y miRNómica dirigida para abordar biomarcadores de enfermedades infecciosas, neurodegenerativas y cáncer

F. Benavente / V. Sanz (UB)

09/2022-08/2025

Ministerio de Ciencia, Innovación y Universidades

Doctorat industrial (Irene Latorre), Investigació i desenvolupament de mètodes analítics per a la determinació d’atributs crítics de qualitat dels PS-liposomes. Estudis d’estabilitat i del procés de liofilització

V. Sanz (UB)

2022-2025

Generalitat de Catalunya, Ahead Therapeutics

2021SGR00681, Bioanàlisi

V. Sanz (UB)

01/2022-6/2025

Generalitat de Catalunya

Doctorat industrial (Oumaina El Ouahabi), Desenvolupament i validació de noves metodologies analítiques per a la caracterització i control de qualitat de liposomes per al tractament de malalties autoimmunes

V. Sanz (UB)

2021-2024

Generalitat de Catalunya, Ahead Therapeutics

XaRTA, Xarxa de Referència d’R+D+I en Tecnologia dels Aliments, Generalitat de Catalunya

C. Carretero (UAB)

01/2018-12/2022

Generalitat de Catalunya

RTI2018-097411-B-I00, Desarrollo de estrategias analíticas innovadoras para el estudio de biomarcadores proteómicos, metabolómicos y miRómicos de enfermedades neurodegenerativas y cáncer

V. Sanz / F. Benavente (UB)

01/2019-06/2022.

Ministerio de Ciencia, Innovación y Universidades

FBG312538, Unveiling bioactive peptides in porcine plasma hydrolysates through fractionation and top-down proteomics characterization, APC Europe S.L.U.

F. Benavente i L. Pont (UB), 02/2024-04/2025

FBG312253, Identification of bioactive peptides in proteomics maps of pig plasma hydrolysates, APC Europe S.L.U.

F. Benavente / L. Pont Villanueva (UB), 05/2023-11/2023

FBG311893, Fractionation and top-down proteomics characterization of pig plasma hydrolysates, APC Europe S.L.U.

F. Benavente / L. Pont (UB), 09/2022-04/2023

FBG312405, Establiment de mètodes per a la caracterització de liposomes, Ahead Therapeutics

V. Sanz (UB) 03/2023-02/2024

FBG311745, Establiment de mètodes per a la caracterització de liposomes, Ahead Therapeutics

V. Sanz (UB) 03/2022-02/2023

FBG311724, Estudio de la distribución del tamaño de partícules de PS-liposomas mediante difracción làser

V. Sanz / F. Benavente (UB) 01/2022-01-2023

FBG311131, Desenvolupament de metodologies per a l’anàlisi de liposomes, Ahead Therapeutics

V. Sanz (UB) 03/2021-02/2022

FBG311184, Análisis de aromes y hemoglobina en hemoderivados de uso alimentario, APC Europe S.L.U.

J. Barbosa / F. Benavente (UB) 03/2021-08/2021

FBG31103, Determinación del tamaño de partícula de liposomes

J. Barbosa / V. Sanz (UB) 01/2021-01/2022

FBG310693, Desenvolupament de metodologies analítiques, Ahead Therapeutics

V. Sanz (UB) 03/2020-02/2021

FBG310493, Characterization of proteolytic enzymes by SEC and MALDI-TOF-MS, APC Europe S.L.U.

J. Barbosa / F. Benavente (UB) 09/2019-03/2020

Human recombinant hyposialylated erythropoietin, methods of purification and therapeutic uses thereof

Autors: Rodríguez, T.; Amaro, D.; García-Artalejo, J.A.; Sosa Testé, I.; Sarmiento Conde, Y.; Hernández de la Rosa, L.; Díaz Goire, D.; Giménez, E.

PCT/CU2020/050001 (WO2021/043345A1), Cuba (5/9/2019) Centro de Inmunología Molecular (La Habana, Cuba)

2019-2021

Desarrollo de estratègies analíticas avanzadas para la caracterización de las proteïnes de quinoa. Aplicación en variedades, tipus de producción agrícola y tecnologies de procesamiento

Dir: F. Benavente / V. Sanz

Setembre 2024

New strategies based on mass spectrometry for the sensitive analysis of protein and microRNA biomarkers in human biofluids

Dir. F. Benavente / E. Giménez

Juliol 2024

Separación y caracterización de glicopéptidos y glicanos en fluidos biológicos. Aplicación al diagnóstico del cáncer y otras patologías

Dir. V. Sanz / E. Giménez

Desembre 2020

Nuevas estrategias para la mejora de la sensibilidad y la selectividad en el análisis de biomarcadores proteómicos y mirNómicos en fluídos biológicos mediante CE-MS

Dir. V. Sanz / F. Benavente

Premi extraordinari de doctorat (UB)

Premi millor tesi SECyTA (2020)

Novembre 2020

Detección y caracterización de péptidos, proteínas y glicoproteínas como biomarcadores en fluidos biológicos. Aplicación al diagnóstico y seguimiento de diversas patologías

Dir: V. Sanz / F. Benavente

Premi extraordinari de doctorat (UB)

Juliol 2017

Desarrollo de metodologías analíticas para la separación, detección y caracterización de biomarcadores glicoproteicos en muestras biològicas

Dir. V. Sanz i E. Giménez

Setembre 2017