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Innovation and improvement of bacterial culture media

Innovation and improvement of bacterial culture media
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Innovation and improvement of microbiological culture media used in the analysis of food, medicines, water and clinical samples.

Our research group collaborates with the company Reactivos para Diagnóstico, SL, which manufactures microbiological culture media. In collaboration with this company, two industrial doctorates have been carried out: “Innovation in the detection of Legionella and in the cultivation of demanding bacteria” and “Innovation in modular technology for the mass sowing of microbiological culture media” and 6 scientific articles have been published.

Investigador principal
https://orcid.org/0000-0002-7308-030X
Equip

Casino P, López A, Peiró S, Rios S, Porta A, Agustí G, Terlevich D, Asensio D, Marqués AM, Piqué N

Polyethersulfone (PES) filters improve the recovery of Legionella spp. and enhance selectivity against interferint microorganisms in water samples

Polymers; DOI: https://doi.org/10.3390/polym15122670

Casino P, López A, Peiró S, Terrones I, Agustí, G, Terlevich D, Asensio D, Marqués AM, Piqué N

Use of Blood Powder (Ground and Irradiated) for the Manufacture of Chocolate Agar

Int J Mol Sci; DOI: https://10.3390/ijms24097965

Terrones-Fernandez I, Casino P, López A, Peiró S, Ríos S, Nardi-Ricart A, García-Montoya E, Asensio D, Marqués AM, Castilla R, Gamez-Montero PJ, Piqué N

Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration

Microbiol Spectr; DOI: https://10.1128/spectrum.03161-22

Torrico M, Casino P, López A, Peiró S, Rios M, Rios S, Montes MJ, Guillén C, Nardi-Ricart A, García-Montoya E, Asensio D, Marqués AM, Piqué N

Improvement of Mueller-Kauffman Tetrathionate-Novobiocin (MKTTn) enrichment medium for the detection of Salmonella enterica by the addition of ex situ-generated tetrathionate

J Microbiol Methods; DOI: https://10.1016/j.mimet.2022.106524

Casino, P., López, A., Peiró, S., Ríos, M., Ríos, S., Porta, A., Agustí, G., Asensio, D., Marqués, A. M., & Piqué, N

GVPC Medium Manufactured without Oxygen Improves the Growth of Legionella spp. and Exhibits Enhanced Selectivity Properties

Microbiology spectrum; DOI: https://10.1128/spectrum.02401-21

Terrones-Fernandez I, Rodero-De-Lamo L, López A, Peiró S, Asensio D, Castilla R, Gamez-Montero PJ, Piqué N.

Microwave oven application for the preparation and sterilization of microbiological culture media: a feasible method with an adapted water bath and perforable cap

Appl Sci; DOI: https://doi.org/10.3390/app14062340

; DOI:

Disseny i millora de medis de cultiu microbiològics quant a selectivitat, agents solidificants i nutrients

https://https://www.reactivosparadiagnostico.com/

Núria Piqué Clusella

2020

Projectes de millora de medis de cultiu microbiològics en col·laboració amb l’empresa Reactivos para Diagnóstico, SL

Innovación en la detección de Legionella y en el cultivo de bacterias exigentes

In this thesis, the properties of the GVPC medium for the isolation of Legionella spp. in water samples were improved by modifying the sterilisation conditions and the elimination of oxygen during manufacture.

The best membrane filter for the detection of Legionella in water samples was also identified.

A chromogenic medium for Legionella was developed, based on two chromogens containing short and long chain fatty acids.

The chocolate agar medium was modified with the addition of a preparation of bovine blood powder, commonly used for veterinary use. The crushed and irradiated product resulted in an alternative medium to chocolate agar, with a higher content of non-lysed erythrocytes.

2024