Agrobiotechnology applied to crop resilence: biostimulants and biocontrol (AGRO-BIO)

Agrobiotechnology applied to crop resilence: biostimulants and biocontrol (AGRO-BIO)

Our research focuses on developing strategies to improve crop resilience to both biotic and abiotic stresses. To this end, we investigate plant metabolism with a particular focus on polyamine metabolism and metabolite-mediated signaling, as well as the interactions between plants and rhizospheric microorganisms that enhance crop performance and stress tolerance.

Our work spans from fundamental research aimed at deciphering the molecular functions of polyamines and their signaling pathways, to applied approaches focused on the development of stress-resilient crop varieties and microbe-based biostimulants and biocontrol agents.

Through this integrated view, we aim to deliver sustainable solutions that strengthen crop productivity and resilience, bridging fundamental research with real-world applications in the agri-food industry.

Dr. Rubén Alcázar Hernández
Investigador principal
Dr. Rubén Alcázar Hernández

I obtained a Bachelor’s degree in Pharmaceutical Sciences from the University of Barcelona (UB) in 1999 and a Ph.D. in Plant Biology from the same institution in 2004. Following my doctoral studies, I carried out postdoctoral research at the University of Barcelona (2004–2005) and later as a senior postdoctoral researcher at the Max Planck Institute for Plant Breeding Research (Cologne, Germany), in a research line which involved the Department of Plant Breeding and Genetics and the Department of Plant-Microbe Interactions (2006–2012).

In 2012, I joined the University of Barcelona as a Ramón y Cajal researcher (2012–2017) where I established my independent research group, and I was awarded a Marie Curie Career Integration Grant as start-up suport (2012–2016). Since, I have been PI of several fundamental and transfer of Technology projects and co-inventor of a patent for a plant biostimulant protecting crops against stress. Since 2017, I am Associate Professor at the University of Barcelona. I have served as board of the Spanish Society of Plant Biology (SEBP). During my research, I have specialized in the areas of plant molecular biology and genomics, metabolomics, quantitative genetics, genetics of natural variation, plant–microorganism interactions, and mechanisms of tolerance to abiotic stresses (cold/freezing, salinity, and drought) in plants.

https://orcid.org/0000-0002-3567-7586
Equip
Línies de recerca
  • Polyamines and crop disease resistance

    In this research line, polyamine signaling is investigated to improve crop resistance to necrotrophic and hemibiotrophic pathogens. By dissecting their role in cell death and defense signaling, the project develops resistant tomato lines and sustainable, polyamine-based plant biostimulants.

  • Microbial-based solutions to enhance crop resilience

    We design and develop next-generation biostimulants and biopesticides based on multifunctional plant-associated microorganisms to promote plant growth, yield, and fruit quality, while enhancing tolerance to abiotic stresses (e.g., drought, salinity, cold/freezing) and strengthening plant defenses against diverse pathogens, including fungal, bacterial, and parasitic species.

Murillo E, Martínez-Seidel F, Atanasov KE, Gentry-Torfer D, Pereira Firmino AA, Erban A, Nie S, Leeming MG, Suwanchaikasem P, Boughton BA, Williamson NA, Roessner U, Kopka J, Alcázar R (2025)

Polyamines and flg22 reshape the ribosomal protein composition of actively translating ribosomes in plants.

Plant Physiol Biochem.220:109585. (2025); DOI: https://doi.org/10.1016/j.plaphy.2025.109585

Díaz-Narváez, Atanasov KE, Murillo E, Alcázar R (2024).

Pararhizobium sp. strains enhancing chilling stress tolerance and yield in tomato plants

Plant Stress 14: 100587 (2024). ; DOI: https://doi.org/10.1016/j.stress.2024.100587

Zhang C., Atanasov KE, Murillo E, Vives-Peris V, Zhao J, Deng C, Gómez-Cadenas A, Alcázar R (2023)

Spermine deficiency shifts the balance between jasmonic acid and salicylic acid-mediated defence responses in Arabidopsis.

Plant, Cell & Environ. 46:3949-3970 (2023). ; DOI: https://doi.org/10.1111/pce.14706

Zhang C., Atanasov K.E., Alcázar R. (2023)

Spermine inhibits PAMP-induced ROS and Ca 2+ burst and reshapes the transcriptional landscape of PTI in Arabidopsis.

J Exp Bot. 74: 427-442 (2023). ; DOI: https://doi.org/10.1093/jxb/erac411

Platre MP, Satbhai SB, Brent L, Gleason MF, Cao M, Grison M, Glavier M, Zhang L, Gaillochet C, Goeschl C, Giovannetti M, Enugutti B, Neveu J, von Reth M, Alcázar R, Parker JE, Vert G, Bayer E, Busch W. (2022)

The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plants.

Nature Commun. 13: 4445 (2022). ; DOI: https://doi.org/10.1038/s41467-022-32167-6

Panahirad S, Gohari G, Mahdavinia G, Jafari H, Kulak M, Fotopoulos V, Alcázar R, Dadpour M. (2023)

Foliar application of chitosan-putrescine nanoparticles (CTS-Put NPs) alleviates cadmium toxicity in grapevine (Vitis vinifera L.) cv. Sultana: modulation of antioxidant and photosynthetic status.

BMC Plant Biol. 23:411 (2023). ; DOI: https://doi.org/10.1186/s12870-023-04420-7

Development of soil bacteria based plant growth.

2021

Development of soil bacteria based plant growth. F2I-FVal_2020-002. (07/01/2021-06/12/2021). Fundació Bosch i Gimpera de la Universitat de Barcelona / Fundació Bosch i Gimpera de la Universitat de Barcelona IP:Rubén Alcázar Hernández

DESARROLLO DE PRODUCTOS BIOESTIMULANTES

DESARROLLO DE PRODUCTOS BIOESTIMULANTES BASADOS EN MICROORGANISMOS PARA LA PROTECCIÓN FRENTE AL ESTRÉS ABIÓTICO EN CULTIVOS. (01/12/2021-31/05/2024). Ref. PDC2021-121267-I00. Ministerio de Ciencia e Innovación / Ministerio de Ciencia e Innovación IP:Rubén Alcázar Hernández

CONTRIBUCIÓN DE LAS POLIAMINAS A LA RESPUESTA SISTÉMICA ADQUIRIDA

CONTRIBUCIÓN DE LAS POLIAMINAS A LA RESPUESTA SISTÉMICA ADQUIRIDA (SAR) Y A LA SEÑALIZACIÓN SOBRE LA INTEGRIDAD DE LA PARED CELULAR (CWI) EN PLANTAS. (01/09/2022-31/08/2025). Ref. PID2021-126896OB-I00. Ministerio de Ciencia e Innovación / Ministerio de Ciencia e Innovación IP:Rubén Alcázar Hernández

Nuevas estrategias de biocontrol de nemátodos con microorganismos rizosféricos

Nuevas estrategias de biocontrol de nemátodos con microorganismos rizosféricos (RizoNem). (02/12/2024-01/12/2027). Ref. CPP2023-010476. Ministerio de Ciencia e Innovación / Ministerio de Ciencia e Innovación IP:Rubén Alcázar Hernández

Pararhizobium-based plant biostimulant improving abiotic stress tolerance in crops.

Pararhizobium-based plant biostimulant improving abiotic stress tolerance in crops. F2I-PdC_2024-015. (13/01/2025-12/01/2026). Fundació Bosch i Gimpera de la Universitat de Barcelona / Fundació Bosch i Gimpera de la Universitat de Barcelona IP:Rubén Alcázar Hernández

Pararhizobium-based plant biostimulant improving abiotic stress tolerance.

Pararhizobium-based plant biostimulant improving abiotic stress tolerance. F2I-MiR_2024_003. (14/03/2025-13/09/2025). Fundació Bosch i Gimpera de la Universitat de Barcelona / Fundació Bosch i Gimpera de la Universitat de Barcelona IP:Rubén Alcázar Hernández

Modulación de la muerte celular y la defensa vegetal por poliaminas en respuesta a patogenos con contrastadas estrategias de infección.

Modulación de la muerte celular y la defensa vegetal por poliaminas en respuesta a patogenos con contrastadas estrategias de infección. (01/09/2025-31/08/2028). Ref. PID2024-157049OB-I00. Ministerio de Ciencia e Innovación / Ministerio de Ciencia e Innovación IP:Rubén Alcázar Hernández

Pararhizobium strains, compositions and uses thereof as plant growth stimulants

 

Inventors: Alcázar R, Atanasov KE.

Type: EU Patent  Status: Granted

Application number: 23382666.8.                  PCT/EP2024/067302

Title: Pararhizobium strains, compositions and uses thereof as plant growth stimulants

Priority countries: EUROPEAN UNION / UNITED STATES

Priority Date: 29/06/2023

Owner entities: Universitat de Barcelona

2023

Evaluación de un protocolo de micropropagación de yuca (Manihot esculenta crantz) de cultivares reina y chirosa en el departamento de Córdoba.

Lucía Candelaria Díaz Narváez, PhD fellow Ministerio de Ciencias Colombia (2021-2025)

2025

The Contribution of the Polyamine Spermine to Plant Defense 

Chi Zhang, PhD fellow China Scholarship Council CSC (2020-2024)

2024

Involvement of Polyamines in Ribosome Biogenesis and Identification of Potential Genes Associated with Spermine Resistance in Arabidopsis thaliana

Ester Murillo Villuendas, PhD fellow FPI (2019-2023)

2023

Polyamine metabolism during the defense response to Pseudomonas syringae in Arabidopsis thaliana

Nazanin Arafaty, PhD fellow APIF-UB (2017-2021)

2021

Involvement of Polyamines in PAMP-triggered Immunity and Systemic Acquired Resistance (SAR). Extragenic Suppressors of Immune Hybrid Incompatibility

Changxin Liu, PhD fellow China Scholarship Council CSC (2016-2020).

2017

Genetics of natural variation and environmental modulation of immune-related hybrid incompatibilities in Arabidopsis

Kostadin E. Atanasov, PhD fellow FPI (Ministry of Science and Innovation, Spain) (2016-19).

2019